. Clinica e batteriologia ematologia per professionisti. Batteriologia; ematologia. Morfologia dei leucociti e globuli rossi del metodo 221.-Il paziente si punga e la prima goccia di sangue spazzato via come prima. Una delle metà delle strisce di sigaretta-carta è ora tenuto in mano destra, l'index-dito è posto al di sopra del nastro e i bordi tenuta tra pollice e indice e del dito indice e medio dita rispettivamente; questo lo converte in una grondaia, il bordo convesso di che è verso il basso. Il bordo di questo canale di scolo che punti lontano da voi (e che è formata da una macchina a bordo di taglio
2762 x 905 px | 23,4 x 7,7 cm | 9,2 x 3 inches | 300dpi
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. Clinical bacteriology and haematology for practitioners. Bacteriology; Hematology. MORPHOLOGY OF LEUCOCYTES AND RED CORPUSCLES 221 Method.—The patient is pricked, and the first drop of blood wiped away as before. One of the half strips of cigarette-paper is now held in the right hand, the index-finger being placed above the strip, and the edges held between the thumb and index-finger and the index and middle fingers respectively; this converts it into a gutter, the convex edge of which is downward. The edge of this gutter which points away from you (and which is formed by a machine-cut edge of the paper) is now dipped into the drop of blood, and a small quantity picked up on its lower surface. This lower surface is then placed on a clean slide parallel to one of its shorter edges and about ^ inch from it, and pressed gently upon it so as to flatten out the paper gutter; as this flattens out the edge of the drop of blood on its under surface will follow it. The strip of paper is now drawn towards the other. Fig. 49.—Method of spreading Films with Cigarette-paper. end of the slide with a steady uniform movement, and in doing so the drop of blood is spread out into a long uniform film. In this way a film inch wide and 2 inches long can be made on a single slide. A fresh piece of paper is to be used for each specimen (see Fig. 49). The author is of opinion that it is best to adopt the cover-glass method, as he has found that it presents fewer difficulties for beginners; this' is not the universal experience, and it is a good plan to try both, and adopt that with which you get the best results. Films are sometimes spread on one slide by means of another, which is used as a spreader in much the same way as the cigarette-paper described above. This is very good for malaria parasites, and for alterations in the red corpuscles, but is useless for making differential counts, as some of the leucocytes are carried along with the spreader and left at the end of the film.
