. Il bollettino biologico. Biologia; Zoologia; biologia; biologia marina. 0,20-1 e 0,15- o o CD o o il controllo PP 1 mg / ml 0,10- 0,05 - 0,00. 20 30 40 Minuti 0 10 20 30 40 50 60 minuti di figura 3. Time curve di risposta dopo 2 h (A), 16 h (B), 40 h (C). e 64 h (D) di fame. La ODs della sospensione cellulare sono stati 0,19, 0,20, 0,13 e 0,12, rispettivamente, e la massima ODs in risposta al PP ad una concentrazione di 1 mg ml' (A) sono state di 0,18 (20 min), 0,27 (22 min), 0,17 (38 min). e 0,13 (24 min), rispettivamente. Che è il numero massimo di risposte assolute di peptone proteasi (PP) erano 63. 92, 85, e 77%,

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 0.20-1 E 0.15- o o CD o o Control PP 1 mg / ml 0.10- 0, 05 - 0.00. 20 30 40 Minutes 0 10 20 30 40 50 60 Minutes Figure 3. Time-response curves after 2 h (A), 16 h (B), 40 h (C). and 64 h (D) of starvation. The ODs of the cell suspension were 0.19, 0.20, 0.13, and 0.12, respectively, and the maximum ODs in response to PP at a concentration of 1 mg ml~' (A) were 0.18 (20 min), 0.27 (22 min), 0.17 (38 min). and 0.13 (24 min), respectively. That is. the maximum absolute responses to protease peptone (PP) were 63. 92, 85, and 77%, respectively. The background—control with no attractant (D)—was drastically reduced after 40 h of starvation in comparison to the background obtained after a shorter period of starvation. The assay was sensitive to weak attractants only between 16 and 64 h of starvation, and the most distinguishable and reproducible quantitative determinations were obtained using cells starved for 40 h. Cells with maximal sensitivity could be obtained within a shorter period of starvation if cells were grown to the early stationary phase before they were transferred to star- vation medium. Thus, the results shown in Figure 4 and Table I are from experiments using early stationary phase cells starved for 24 h. The time-response curves offer three approaches for quantitative analysis: (1) initial slope of the absorbance versus time, (2) final maximal absorbance within the time period of measurement, and (3) signal-to-noise ra- tio (S/N) at a fixed time—i.e.. S/N = 1, no chemoki- nesis; S/N 1, positive chemokinesis. In the experiments shown in Figure 4 and Table I, we tested a variety of animal peptide hormones, some of which have previously been found to work as che- moattractants for Tetrakymena. Table I lists the S/N's obtained after 24 min; Figure 4 shows representative time-response curves for a number of attractants tested. Obviously, /^-endorphin, FGF, insulin, and PDGF all are good

Parole chiave associate all'immagine

• bookauthorlilliefrankrat
• booksubjectbiology
• booksubjectzoology