. Il bollettino biologico. Biologia; Zoologia; biologia; biologia marina. 386 M. XIJHOUT E L. M. RIDDIFORD indipendente JH O JH-processo controllato. È probabile che il periodo di cambio JH-indipendenza è effettivamente più breve poiché è necessario un tempo finito per il declino del sangue del titolo di JH. Effetto di allatccton/y su vitellogenin concentrazione in vista del fatto che diversi insetti maturazione ovarica sistemi richiedono JH per la sintesi della femmina-specifica proteina di tuorlo, era di interesse per stabilire la presenza di un tale vitellogenin in Manduchi e determinare l'effetto di alla-

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. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 386 M. M. XIJHOUT AND L. M. RIDDIFORD independent of JH or of the JH-controlled process. It is probable that the period of JH-independence is actually shorter since a finite time is required for the decline of the blood titer of JH. Effect of allatccton/y on vitellogenin concentration In view of the fact that several insect ovarian maturation systems require JH for synthesis of the female-specific yolk protein, it was of interest to establish the presence of such a vitellogenin in Manduca and to determine the effect of alla- tectomy on its concentration in the blood and uptake into follicles. cu "o i_ Q.. -6 -4 -2 0 +2 +4 Days of adult life FIGURE 5. Concentration of vitellogenin in blood during development. Each point represents the mean value of 5 samples. Error bars show standard deviation. When blood samples of early pupae and freshly-emerged adults were subjected to SDS gel electrophoresis, a sex-specific protein band was found in the adult female (see Figure 4). The same band was found to be a major protein component of the mature egg. This is, presumably, the vitellogenin protein. In order to determine the time in development when the vitellogenin first ap- peared in the blood, hemolymph samples were withdrawn from developing adults at 3 day intervals after pupal ecdysis. No vitellogenin was found in animals imme- diately after pupation nor during early adult development. In the 18 day period between pupal ecdysis and adult eclosion, vitellogenin was first detected in the blood with this technique 3 days before eclosion. During adult life, as shown in Figure 5, the yolk protein comprised approximately 15% of total blood protein which stained with Coomassie Brilliant Blue. This stain is quite sensitive and will detect less than 0.5 /u, g of protein in a single band (Weber, ct al.. 1972).. Please note that these images are extracted from scanned page images that may have been digitally en

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• bookauthorlilliefrankrat
• booksubjectbiology
• booksubjectzoology